Advanced Multiplex PCR Assay for Differentiation of Brucella Species

作者:Kang Sung Il; Her Moon*; Kim Jong Wan; Kim Ji Yeon; Ko Kyung Yuk; Ha Yun Mi; Jung Suk Chan
来源:Applied and Environmental Microbiology, 2011, 77(18): 6726-6728.
DOI:10.1128/AEM.00581-11

摘要

Two new primer sets of a 766- and a 344-bp fragment were introduced into the conventional Bruce-ladder PCR assay. This novel multiplex PCR assay rapidly and concisely discriminates Brucella canis and Brucella microti from Brucella suis strains and also may differentiate all of the 10 Brucella species.

  • 出版日期2011-9