Beyond biocompatibility: an approach for the synthesis of ZnS quantum dot-chitosan nano-immunoconjugates for cancer diagnosis

作者：Mansur Herman S^*; Mansur Alexandra A P; Soriano Araujo Amanda; Lobato Zelia I P

来源：Green Chemistry, 2015, 17(3): 1820-1830.

DOI：10.1039/c4gc02072c

In this study, we designed and developed novel biocompatible ZnS quantum dot (QD) nano-immunoconjugates to detect cancer cells strictly via an environmentally-friendly chemistry process. ZnS QDs were capped by chitosan-based ligands that were chemically conjugated at room temperature with an antibody-specific cancer biomarker for non-Hodgkin lymphoma using a one-pot aqueous colloidal route. The nano-immunoconjugates were extensively characterised by several spectroscopic and morphological approaches and biological assays. The results demonstrated that ultra-small colloidal ZnS nanocrystals with an average diameter of 3.7 nm were produced and stabilised by the chitosan-antibody conjugates. In addition, they exhibited intense fluorescence activity and were effective for specific targeting, labelling, and bioimaging of cancerous lymphocyte B-cells. Moreover, the results of in vitro cell viability assays using fibroblast cell line indicated that the ZnS-nanoconjugates were cytocompatible. These may be used in numerous applications in oncology diagnosis and nanomedicine.

• 出版日期2015