科研之友
微信
新浪微博
Facebook
分享链接
摘要
Human papillomavirus (HPV) is now considered as a causative agent of carcinoma of the uterine cervix. Papillomaviruses are a diverse group of DNA-based viruses that infect the skin and mucous membranes of humans and a variety of animals. Over 100 different human papillomavirus (HPV) types have been identified. Genital human papillomaviruses are classified into low-risk and high-risk. The low-risk HPVs are associated with genital warts and lesions. Persistent infection with a subset of about 13 so-called "high-risk" sexually transmitted HPVs may lead to the development of cervical intraepithelial neoplasia (CIN), vulvar intraepithelial neoplasia (VIN), penile intraepithelial neoplasia (PIN), and/or anal intraepithelial neoplasia (AIN). These are precancerous lesions and can progress to invasive cancer. HPV infection is a necessary factor in the development of nearly all cases of cervical cancer. The main methods in HPV diagnosis are molecular methods. In this research we used Roche's LINEAR ARRAY HPV Genotyping Test (CE-IVD). This method combines multiplex PCR with 40 pairs of primers and DNA hybridization. The products from the PCR are used to hybridize with nylon strips which contain immobilized DNAs of 38 types and subtypes of HPV genotypes. The cervical smears were collected from Bulgarian patients with condylata acuminate lesions, in three hospitals in Sofia-University Hospital of Obstetrics and Gynaecology "Maichin Dom", "Tokuda" Hospital and the "National Institute of Oncology and Hematology". One hundred and twenty six cervicovaginal smears and scrapings were tested by this method. Forty two percent of them were found positive for HPV DNA and fifty eight percent, negative. We found more than one type of viral DNA in all positive samples. We found HPV 16 DNA in twenty two samples (38%); HPV 18 DNA, in eight samples (18%); HPV 6 DNA, in five cases (10%); HPV 53 DNA, in seven cases (11%); HPV 31 DNA, in three cases (4%); HPV 35, in four cases (5%), and in two cases we found viral DNA types HPV 61 and HPV 62. Further studies are needed to determine the influence these HPV infections have on the epidemiology of the genital tract of these patients.
- 出版日期2011
