Monitoring patterned enzymatic polymerization on DNA origami at single-molecule level

作者：Okholm A H; Aslan H; Besenbacher F; Dong M^*; Kjems J

来源：Nanoscale, 2015, 7(25): 10970-10973.

DOI：10.1039/c5nr01945a

DNA origami has been used to orchestrate reactions with nano-precision using a variety of biomolecules. Here, the dynamics of albumin-assisted, localized single-molecule DNA polymerization by terminal deoxynucleotidyl transferase on a 2D DNA origami are monitored using AFM in liquid. Direct visualization of the surface activity revealed the mechanics of growth.

• 出版日期2015