alpha-Trideuteromethyl[15N]glutamine: A Long-Lived Hyperpolarized Perfusion Marker

作者：Durst Markus^*; Chiavazza Enrico; Haase Axel; Aime Silvio; Schwaiger Markus; Schulte Rolf F

来源：Magnetic Resonance in Medicine, 2016, 76(6): 1900-1904.

DOI：10.1002/mrm.26104

• hyperpolarized 15N
• perfusion
• kidney

Purpose: We characterized the performance of a novel hyperpolarized perfusion marker, alpha-trideuteromethyl[15N]glutamine, for direct comparison with a 13C-based hyperpolarized perfusion marker, [13C, 15N2] urea.
Methods: A hardware platform and pulse sequence for in vivo 15N experiments were established. Hyperpolarized solutions of alpha-trideuteromethyl[15N] glutamine and [13C, 15N2] urea were injected into healthy male Lewis rats. Kidney slice images were acquired using a single-shot spiral readout. Both compounds were compared to determine in vivo signal lifetime and tracer distribution. Mass spectrometry was performed to evaluate excretion of the compound.
Results: Compared with 13C-labeled urea, a significantly increased signal lifetime was observed. While the urea signal was gone after 90 s, decay of the glutamine compound was sufficiently slow to obtain a quantifiable signal, even after 5 min. The glutamine derivative showed strong localization in the kidneys with little background signal. Effective T1 of alpha-trideuteromethyl[15N]glutamine was approximately eight-fold higher than that of urea. Mass spectrometry results confirmed rapid excretion within the time scale of the measurement.
Conclusion: Hyperpolarized alpha-trideuteromethyl[15N] glutamine is a highly promising candidate for renal studies because of its long signal lifetime, strong localization and rapid excretion.

• 出版日期2016-12