Embryo viability indexing using Raman spectroscopy of spent culture media

作者：Parlatan Ugur; Basar Gunay; Bavili Nima; Yumru Harika; Sag Fatih; Bulgurcuoglu Sibel; Bastu Ercan

来源：Spectroscopy Letters, 2016, 49(7): 458-463.

DOI：10.1080/00387010.2016.1198814

• Band component analysis
• embryo viability
• in vitro fertilization
• Raman spectroscopy

Embryo viability quantification is an important topic for in vitro fertilization researchers. This study suggests Raman spectroscopy as a candidate method to find an objective measure of embryo viability. Raman spectra of 31 spent embryo culture samples (16 nonpregnant, 15 pregnant) from 31 patients were analyzed with band component analysis. The band area ratio of 902 to 943 cm 1 was found to be the most discriminatory. These bands are related to glutamine, glycine, proline, aspartic acid, and valine. According to the pregnancy outcome the sensitivity and specificity of Raman analysis were found to be 93% and 77%, respectively.

• 出版日期2016