Modifications in Rat Plasma Proteome after Remote Ischemic Preconditioning (RIPC) Stimulus: Identification by a SELDI-TOF-MS Approach

作者：Hibert Pierre; Prunier Mirebeau Delphine; Beseme Olivia; Chwastyniak Maggy; Tamareille Sophie; Pinet Florence; Prunier Fabrice^*

来源：PLos One, 2014, 9(1): e85669.

DOI：10.1371/journal.pone.0085669

Remote ischemic preconditioning%26apos;s (RIPC) ability to render the myocardium resistant to subsequent prolonged ischemia is now clearly established in different species, including humans. Strong evidence suggests that circulating humoral mediators play a key role in signal transduction, but their identities still need to be established. Our study sought to identify potential circulating RIPC mediators using a proteomic approach. Rats were exposed to 10-min limb ischemia followed by 5-(RIPC 5%26apos;) or 10-min (RIPC 109) reperfusion prior to blood sampling. The control group only underwent blood sampling. Plasma samples were isolated for proteomic analysis using surface-enhanced laser desorption and ionization - time of flight - mass spectrometry (SELDI-TOF-MS). A total of seven proteins, including haptoglobin and transthyretin, were detected as up-or down-regulated in response to RIPC. These proteins had previously been identified as associated with organ protection, anti-inflammation, and various cellular and molecular responses to ischemia. In conclusion, this study indicates that RIPC results in significant modulations of plasma proteome.

• 出版日期2014-1-13