D-Tagatose production by Lactococcus lactis NZ9000 Cells Harboring Lactobacillus plantarum L-arabinose Isomerase

作者：Zhang, Yao; Fan, Yanli; Hu, Haijie; Yang, Haixu; Luo, Xuegang; Li, Zhongyuan; Zhou, Hao; Ma, Wenjian; Song, Yajian^*; Zhang, Tongcun

来源：Indian Journal of Pharmaceutical Education and Research, 2017, 51(2): 288-294.

DOI：10.5530/ijper.51.2.34

• D-tagatose
• L-arabinose isomerase
• Lactococcus lactis NZ9000
• Lactobacillus plantarum
• Galactose

D-tagatose is a functional sweetener present in medicine, food, and dairy products and with broad market prospects, and L-arabinose isomerase gene (araA) can mediate the bioconversion of D-galactose into D-tagatose. In this study, a Lactococcus lactis NZ9000 strain harboring exogenous L-arabinose isomerase was constructed to produce D-tagatose. Lactobacillus plantarum CGMCC 8198 exhibits L-arabinose isomerase activity and its genome has been sequenced. The araA gene of Lactobacillus plantarum CGMCC 8198 encoding L-arabinose isomerase was identified by sequence analysis and was successfully cloned and expressed in Lactococcus lactis NZ9000. The D-tagatose production by the whole cell of the recombinant strain was optimized. The optimal condition for conversion reaction was at 50 degrees C, pH 7.0 and with 300 mmol/L Mn2+ and 60 g/L galactose added. The D-tagatose yield and conversion rate at the optimal condition was determined and reached 40.2 g/L and 67%, respectively.

• 出版日期2017-4
• 单位天津科技大学