Hydrolytic cleavage of double-strand DNA by the water-soluble dicobalt(III) complexes of 1,4,7-Triazacyclononane-N-acetate

作者：Qian Jing; Ma Xiaofang; Tian Jinlei; Gu Wen; Shang Jing; Liu Xin; Yan Shiping^*

来源：Journal of Inorganic Biochemistry, 2010, 104(9): 993-999.

DOI：10.1016/j.jinorgbio.2010.05.005

• Cobalt(III) complexes
• 1,4,7-triazacyclononane-N-acetate
• mu-Hydroxo
• Hydrolytic cleavage

Three water-soluble dicobalt(III) complexes, [Co2L2(mu-OH)(2)](ClO4)(2)center dot 5H(2)O (1), [Co2L2(mu-OH)(2)](ClO4)(2)center dot-CH3OH center dot H2O(2); [Co2L2(mu-OH)(2)](ClO4)(2)center dot 4H(2)O(3) (L = 1,4,7-triazacyclononane-N-acetate monoanion), were prepared to serve as nuclease mimics. The complexes were characterized by X-ray, IR and UV-vis spectroscopy as well as ESI-MS. Three complexes exhibit similar structures, just with different solvent molecules. The electrospray mass spectrum of 1 in solution indicates that dinuclear ion [Co2L2(mu-OH)(2)-H+](+) (4) is the active species. In the absence of any reducing agent, the complexes cleave plasmid pBR322 DNA was performed and its hydrolytic mechanism was demonstrated with radical scavengers, anaerobic reaction and T4 ligase. The kinetic aspects of DNA cleavage under pseudo- or true-Michaelis-Menten conditions are also detailed, kinetic parameters (k(cat), K-M) were calculated to be 3.57 h(-1), 6.92 x 10(-4) M; 0.28 h(-1), 1.9 x 10(-5) M for 4, respectively.

• 出版日期2010-9
• 单位郑州大学; 南开大学; 天津师范大学