Morphological and physicochemical aspects of microbial lipase obtained from novel agroindustrial waste encapsulated in a sol-gel matrix

作者：Zubiolo Claudia; Andrade Santos Rafaela Cristiane; Figueiredo Renan Tavares; Faria Soares Cleide Mara; Lins de Aquino Santana Luciana Cristina

来源：Journal of Thermal Analysis and Calorimetry, 2015, 120(3): 1503-1509.

DOI：10.1007/s10973-015-4494-3

• Microbial enzyme
• Silica sol-gel
• Entrapped lipase
• Aspergillus niger

The lipase from Aspergillus niger, obtained from solid-state fermentation of pumpkin seed flour, was encapsulated in a sol-gel matrix. The silica obtained by the sol-gel technique and lipase encapsulated were characterised with regard to superficial area, pore volume and size by nitrogen adsorption (BJH method), scanning electron microscopy, mass loss upon heating (TG) and differential scanning calorimetry. The immobilised biocatalyst and the sol-gel matrix showed features typical of mesoporous solids. The presence of the enzyme encapsulated in the sol-gel matrix was confirmed since the total mass loss of the BI at a temperature of 1,000 A degrees C was approximately 60 %, while for the free enzyme the mass loss was 95 %. This new lipase obtained from the fermentation of agroindustrial waste, which when encapsulated in sol-gel has physical-chemical and morphological features similar to those of conventional biocatalysts, maybe exploited for future applications in biocatalytic processes.

• 出版日期2015-6