A preliminary study of the whole-genome expression profile of sporadic and monogenic early-onset Alzheimer%26apos;s disease

作者：Antonell Anna^*; Llado Albert; Altirriba Jordi; Botta Orfila Teresa; Ba**** Mircea; Fernandez Manel; Ferrer Isidre; Sanchez Valle Raquel; Luis Molinuevo Jose

来源：Neurobiology of Aging, 2013, 34(7): 1772-1778.

DOI：10.1016/j.neurobiolaging.2012.12.026

• Microarray
• Expression
• Early-onset Alzheimer%26apos
• s disease
• Familial Alzheimer%26apos
• s disease
• PSEN1 mutation
• Sporadic

Alzheimer%26apos;s disease (AD) is the most common neurodegenerative dementia. Approximately 10% of cases present at an age of onset before 65 years old, which in turn can be monogenic familial AD (FAD) or sporadic early-onset AD (sEOAD). Mutations in PSEN1, PSEN2, and APP genes have been linked with FAD. The aim of our study is to describe the brain whole-genome RNA expression profile of the posterior cingulate area in sEOAD and FAD caused by PSEN1 mutations (FAD-PSEN1). Fourteen patients (7 sEOAD and 7 FAD-PSEN1) and 7 neurologically healthy control subjects were selected and whole-genome expression was measured using Affymetrix Human Gene 1.1 microarrays. We identified statistically significant expression changes in sEOAD and FAD-PSEN1 brains with respect to control subjects (3183 and 3350 differentially expressed genes [DEG] respectively, false discovery rate-corrected p %26lt; 0.05). Of them, 1916 DEG were common between the 2 comparisons. We did not identify DEG between sEOAD and FAD-PSEN1. Microarray data were validated through real-time quantitative polymerase chain reaction. In silico analysis of DEG revealed an alteration in biological pathways related to intracellular signaling pathways (particularly calcium signaling), neuroactive ligand-receptor interactions, axon guidance, and long-term potentiation in both groups of patients. In conclusion, the altered biological final pathways in sEOAD and FAD-PSEN1 are mainly related with cell signaling cascades, synaptic plasticity, and learning and memory processes. We hypothesize that these 2 groups of early-onset AD with distinct etiologies and likely different could present a neurodegenerative process with potential different pathways that might converge in a common and similar final stage of the disease.

• 出版日期2013-7