Direct Competitive Enzyme-Linked Immunosorbent Assay for Detection of Acrylamide in Food Samples

作者：Wu Jing^*; Luo Lin; Xiao Zhi Li; Yang Jin Yi; Sun Yuan Ming; Lei Hong Tao; Shen Yu Dong; Wang Hong; Xu Zhen Lin

来源：Chinese Journal of Analytical Chemistry, 2014, 42(8): 1149-1154.

• Acrylamide
• Polyclonal antibody
• Direct competitive enzyme-linked immunosorbent assay
• Derivatization

Due to the low molecular weight and simple structure, the production of specific antibodies against acrylamide is unavailable. In this study, a novel hapten was synthesized through the derivatization of acrylamide and 4-mercaptophenylacetic acid. The hapten was then coupled to carrier protein and used to immunize New Zealand rabbits. Polyclonal antibody which showed specific binding to the acrylamide derivative (hapten) was obtained. The antibody was labeled with horseradish peroxidase (HRP) and used to develop a direct competitive enzyme-linked immunosorbent assay (dc-ELISA). The dc-ELISA was used to determine the content of acrylamide derivative, and then transferred to the content of acrylamide. The assay showed an IC50 value of 45. 49 mu g/L, a limit of detection of 3. 0 mu g/L and the linear range of 9. 2-195 mu g/L for acrylamide. The recovery of acrylamide from spiked food sample was determined ranging from 83. 6% to 112. 7%. Good correlations between the results of dc-ELISA and standard HPLC-MS/MS were obtained. The proposed dc-ELISA is suitable for the determination of acrylamide in food samples.

• 出版日期2014-8
• 单位华南农业大学