Msd1/SSX2IP-dependent microtubule anchorage ensures spindle orientation and primary cilia formation

作者：Hori Akiko; Ikebe Chiho; Tada Masazumi; Toda Takashi^*

来源：EMBO Reports, 2014, 15(2): 175-184.

DOI：10.1002/embr.201337929

• centrosome
• ciliogenesis
• left-right symmetry
• microtubule anchoring
• spindle orientation

Anchoring microtubules to the centrosome is critical for cell geometry and polarity, yet the molecular mechanism remains unknown. Here we show that the conserved human Msd1/SSX2IP is required for microtubule anchoring. hMsd1/SSX2IP is delivered to the centrosome in a centriolar satellite-dependent manner and binds the microtubule-nucleator -tubulin complex. hMsd1/SSX2IP depletion leads to disorganised interphase microtubules and misoriented mitotic spindles with reduced length and intensity. Furthermore, hMsd1/SSX2IP is essential for ciliogenesis, and during zebrafish embryogenesis, knockdown of its orthologue results in ciliary defects and disturbs left-right asymmetry. We propose that the Msd1 family comprises conserved microtubule-anchoring proteins.

• 出版日期2014-2