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摘要
Objective-To assess a commercially available point-of-care assay for measurement of bovine cardiac troponin I (cTnI) concentration in blood and plasma samples. %26lt;br%26gt;Sample-Prepared bovine plasma standard samples with known concentrations (0 to 1.0 ng/mL) of cTnI and blood and plasma samples obtained from 28 healthy 2.5-month-old Holstein calves. %26lt;br%26gt;Procedures-Coefficients of variation were calculated for concentrations of cTrI in prepared standards determined with the point-of-care assay, and values were compared with the known concentrations. The cTnI concentrations in blood samples obtained from calves determined with the point-of-care assay were compared with cTnI concentrations in plasma samples obtained from those animals determined with a validated immunoassay. %26lt;br%26gt;Results-The coefficients of variation of cTnI concentrations determined for prepared standards by use of the point-of-care assay were low (%26lt; 20%) for standards with cTnI concentrations %26gt;= 0.025 ng/mL. The blood cTnI concentrations determined with the point-of-care assay were not significantly different from the plasma cTnI concentrations determined with the validated immunoassay. %26lt;br%26gt;Conclusions and Clinical Relevance-Results of this study indicated the point-of-care assay had high precision for determination of cTnI concentrations in most evaluated prepared bovine plasma standard samples. The point-of-care assay may be useful for determination of circulating concentrations of cTnI in cattle. (Am J Vet Res 2013;74:870-873)
- 出版日期2013-6
