Accumulation of intraepithelial mast cells with a unique protease phenotype in T(H)2-high asthma

作者：Dougherty Ryan H; Sidhu Sukhvinder S; Raman Kavita; Solon Margaret; Solberg Owen D; Caughey George H; Woodruff Prescott G; Fahy John V^*

来源：Journal of Allergy and Clinical Immunology, 2010, 125(5): 1046-1053.

DOI：10.1016/j.jaci.2010.03.003

• Asthma
• mast cells
• tryptase
• chymase
• carboxypeptidase A3
• stem cell factor

Background: Previously, we found that mast cell tryptases and carboxypeptidase A3 (CPA3) are differentially expressed in the airway epithelium in asthmatic subjects. We also found that asthmatic subjects can be divided into 2 subgroups ("T(H)2 high" and "T(H)2 low" asthma) based on epithelial cell gene signatures for the activity of T(H)2 cytokines.
Objectives: We sought to characterize intraepithelial mast cells (IEMCs) in asthma.
Methods: We performed gene expression profiling in epithelial brushings and stereology-based quantification of mast cell numbers in endobronchial biopsy specimens from healthy control and asthmatic subjects before and after treatment with inhaled corticosteroids (ICSs). We also performed gene expression and protein quantification studies in cultured airway epithelial cells and mast cells.
Results: By means of unsupervised clustering, mast cell gene expression in the airway epithelium related closely to the expression of IL-13 signature genes. The levels of expression of mast cell genes correlate positively with lung function improvements with ICSs. IEMC density was 2-fold higher than normal in subjects with T(H)2-high asthma compared with that seen in subjects with T(H)2-low asthma or healthy control subjects (P = .015 for both comparisons), and these cells were characterized by expression of tryptases and CPA3 but not chymase. IL-13 induced expression of stem cell factor in cultured airway epithelial cells, and mast cells exposed to conditioned media from IL-13 activated epithelial cells showed downregulation of chymase but no change in tryptase or CPA3 expression.
Conclusion: IEMC numbers are increased in subjects with T(H)2-high asthma, have an unusual protease phenotype (tryptase and CPA3 high and chymase low), and predict responsiveness to ICSs. IL-13 stimulated production of stem cell factor by epithelial cells potentially explains mast cell accumulation in TH2-high asthmatic epithelium. (J Allergy Clin Immunol 2010;125:1046-53.)

• 出版日期2010-5