Molecular characterization and heterologous expression of quinate dehydrogenase gene from Gluconobacter oxydans IFO3244

作者：Vangnai A S^*; Promden W; De Eknamkul W; Matsushita K; Toyama H

来源：Biochemistry-Moscow, 2010, 75(4): 452-459.

DOI：10.1134/S0006297910040085

• quinate dehydrogenase gene
• heterologous gene expression
• Gluconobacter oxydans
• pyrroloquinoline quinone

The quinate dehydrogenase (QDH) from Gluconobacter oxydans IFO3244 exhibits high affinity for quinate, suggesting its application in shikimate production. Nucleotide sequence analysis of the qdh gene revealed a full-length of 2475-bp encoding an 824-amino acid protein. The qdh gene has the unusual TTG translation initiation codon. Conserved regions and a signature sequence for the quinoprotein family were observed. Phylogenetic analysis demonstrated relatedness of QDH from G. oxydans to other quinate/shikimate dehydrogenases with the highest similarity (56%) with that of Acinetobacter calcoaceticus ADP1 and lower similarity (36%) with a membrane-bound glucose dehydrogenase of Escherichia coli. The function of the gene coding for QDH was confirmed by heterologous gene expression in pyrroloquinoline quinone-synthesizing Pseudomonas putida HK5.

• 出版日期2010-4