Micrometastasis Volume in Lymph Nodes Determines Disease Recurrence Rate of Stage II Colorectal Cancer: A Prospective Multicenter Trial

作者：Yamamoto Hirofumi^*; Murata Kohei; Fukunaga Mutsumi; Ohnishi Tadashi; Noura Shingo; Miyake Yasuhiro; Kato Takeshi; Ohtsuka Masahisa; Nakamura Yurika; Takemasa Ichiro; Mizushima Tsunekazu; Ikeda Masataka; Ohue Masayuki; Sekimoto Mitsugu; Nezu Riichiro; Matsuura Nariaki; Monden Morito; Doki Yuichiro; Mori Masaki

来源：Clinical Cancer Research, 2016, 22(13): 3201-3208.

DOI：10.1158/1078-0432.CCR-15-2199

Purpose: We reported in a retrospective study that the presence of micrometastasis in lymph nodes, when assessed by carcinoembryonic antigen (CEA)-specific RT-PCR, is a significant prognostic factor in stage II colorectal cancer. The aim of this study was to clarify the clinical value of micrometastasis in a prospective multicenter trial.
Experimental Design: From November 2001 to December 2005, a total of 419 colorectal cancer cases were preoperatively registered at a central data center. Of them, 315 node-negative stage II colorectal cancer cases were enrolled. After RNA quality check, 304 colorectal cancer cases were analyzed for CEA mRNA in lymph nodes by both conventional RT-PCR (a band method) and quantitative RT-PCR. Long-term prognosis of the patients was determined by each method.
Results: A positive band for CEA mRNA was detected in 73 (24.0%) of 304 patients. Postoperative adjuvant chemotherapy was applied in 31 CEA band-positive cases with an oral 5-fluorouracil derivative HCFU (1-hexylcarbamoyl-5-fluorouracil) for 1 year, whereas chemotherapy was not administered to CEA band-negative group. Multivariate Cox regression analyses revealed that a high micrometastasis volume (high MMV, n = 95) was an independent poor prognostic factor for 5-year disease-free survival (DFS; P = 0.001) and 5-year overall survival (OS; P = 0.016).
Conclusions: This prospective clinical trial demonstrates that micrometastasis volume is a useful marker in identifying patients who are at high or low risk for recurrence of stage II colorectal cancer.

• 出版日期2016-7-1
• 单位中国医学科学院肿瘤医院