Differences in innate IFN gamma and IL-17 responses to Bordetella pertussis between BALB/c and C57BL/6 mice: role of gamma delta T cells, NK cells, and dendritic cells

作者：Mosley Yung Yi C; Lu Fangjia; HogenEsch Harm^*

来源：Immunologic Research, 2017, 65(6): 1139-1149.

DOI：10.1007/s12026-017-8957-4

• Bordetella pertussis
• gamma delta T cells
• IFN gamma
• IL-17
• NK cells

Cell-mediated immune responses characterized by the secretion of IFN gamma and IL-17 play an important role in the immune response to Bordetella pertussis (B. pertussis). We investigated innate sources of IFN gamma and IL-17 upon stimulation of spleen cells from BALB/c (B/c) and C57BL/6 (B6) mice with heat-killed B. pertussis (hkBp). Spleen cells from B/c mice secreted less IFN gamma and more IL-17 than those from B6 mice. Innate IFN gamma was produced predominantly by NK cells in B/c mice and by CD8 T cells and NK cells in B6 mice. Innate IL-17 was produced primarily by gamma delta T cells in both mouse strains. The secretion of IFN gamma was abrogated by anti-IL-12, and the production of IL-17 was abolished by anti-IL-1 beta- and anti-IL23-neutralizing antibodies. B/c dendritic cells (DCs) stimulated with hkBp secreted significantly more IL-1 beta and less IL-12 than B6 DCs. Differences in JNK phosphorylation in DCs suggest that this pathway plays a role in the differences between B/c and B6 strains. Mixed cultures of DCs and gamma delta T cells from B/c and B6 showed that cytokines from DCs as well as gamma delta T cell-intrinsic factors contributed to the robust innate IL-17 response in B/c strain. Stimulation of gamma delta T cells with IL-1 beta and IL-23 was sufficient for IL-17 secretion whereas IL-12 inhibited the secretion of IL-17. A larger fraction of gamma delta T cells were gamma delta T-17 cells in B/c mice than B6 mice. Our data indicate important roles for genetically determined factors in the innate IFN gamma and IL-17 responses to B. pertussis.

• 出版日期2017-12