An integrated course based on two dimensional-electrophoresis and mass spectrometry analysis to teach proteomic techniques for undergraduate students

作者：Sha, Shanshan; Jia, Liqiu; Xin, Yi; Yao, Weili; Ma, Yufang^*

来源：Biochemistry and Molecular Biology Education, 2018, 46(4): 354-360.

DOI：10.1002/bmb.21128

• Experimental course
• proteome
• two dimensional-electrophoresis
• mass spectrometry

Here, we developed an integrated course based on two dimensional-electrophoresis and spectrometry mass (2DE-MS) technique for undergraduate students to help them learn proteomic techniques. The soluble proteins in wild type and gene knockout bacteria were separated by 2DE and the differently expressed proteins were identified by MS analysis. The proteomic data was finally confirmed by RT-PCR detection. The separated experiments of 2DE, MS, RNA isolation, RT-PCR, as well as essential bioinformatic analysis, were integrated into a one-week course, which provided students an opportunity to systematically understand the proteomic techniques and their applications in current scientific research.

• 出版日期2018-8
• 单位大连医科大学