A Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase/Poly(L-leucine)/Polydopamine Modified Glassy Carbon Electrode

作者：Zheng, Xinyu; Guo, Yuheng; Zheng, Jinshuai; Ma, Cong; Zhou, Xuechou; Lin, Jiandi; Lin, Ruiyu^*

来源：Russian Journal of Electrochemistry, 2017, 53(5): 443-451.

DOI：10.1134/S1023193517050172

• hydrogen peroxide
• horseradish peroxidase
• poly-L-leucine
• polydopamine
• modified electrode

A simple and practical sensor of hydrogen peroxide (H2O2) was designed successfully. The mixture of horseradish peroxidase (HRP) and chitosan (Chit) are effectively immobilized on the surface of poly-L-leucine/polydopamine modified glassy carbon electrode (PL-LEU/PDA/GCE). Under the optimum conditions, the biosensor based on HRP exhibits a fast amperometric response (within 3 s) to H2O2. The linear response range of the sensor is 0.5-952.0 mu mol L-1, with the detection limit of 0.1 mu mol L-1 (S/N = 3) and the sensitivity of 0.23 A L moL(-1) cm(-2). The apparent Michaelis-Menten constant (k (M) (app)) of the biosensor is evaluated to be 0.12 mmol L-1, which suggests that the HRP-Chit/PL-LEU/PDA/GCE shows a higher affinity for H2O2. The sensor exhibits good sensitivity, selectivity, stability and reproducibility. The proposed method has been successfully applied to the determination of H2O2 in practical samples.

• 出版日期2017-5
• 单位福建农林大学