A Simple and Robust Method for Establishing Homogeneous Mouse Epiblast Stem Cell Lines by Wnt Inhibition

作者：Sugimoto Michihiko; Kondo Masayo; Koga Yumiko; Shiura Hirosuke; Ikeda Rieko; Hirose Michiko; Ogura Atsuo; Murakami Ayumi; Yoshiki Atsushi; Lopes Susana M Chuva de Sousa; Abe Kuniya^*

来源：Stem Cell Reports, 2015, 4(4): 744-757.

DOI：10.1016/j.stemcr.2015.02.014

Epiblast stem cells (EpiSCs) are pluripotent stem cells derived from epiblasts of postimplantation mouse embryos, and thus provide a useful model for studying "primed" pluripotent states. Here, we devised a simple and robust technique to derive high-quality EpiSCs using an inhibitor of WNT secretion. Using this method, we readily established EpiSC lines with high efficiency and were able to use whole embryonic portions without having to separate the epiblast from the visceral endoderm (VE). Expression analyses revealed that these EpiSCs maintained a homogeneous, undifferentiated status, yet showed high potential for differentiation both in vitro and in teratomas. Unlike EpiSCs derived by the original protocol, new EpiSC lines required continuous treatment with the Wnt inhibitor, suggesting some intrinsic differences from the existing EpiSCs. The homogeneous properties of this new version of EpiSCs should facilitate studies on the establishment and maintenance of a "primed" pluripotent state, and directed differentiation from the primed state.

• 出版日期2015-4-14
• 单位RIKEN