A thermostable pyrimidine nucleoside phosphorylase from Brevibacillus borstelensis LK01 for synthesizing halogenated nucleosides

作者：Liu, Ke; Zhou, Youzhi; Zhang, Jinsong; Chu, Jianlin; Zhang, Yewei^*; He, Bingfang^*

来源：Biotechnology Letters, 2017, 39(12): 1903-1910.

DOI：10.1007/s10529-017-2423-1

• Gene mining
• Halogenated nucleoside
• Pyrimidine nucleoside phosphorylase

To isolate a thermostable pyrimidine nucleoside phosphorylase (PyNP) from mesophilic bacteria by gene mining.
BbPyNP from Brevibacillus borstelensis LK01 was isolated by gene mining. BbPyNP had a highest 60% identity with that of reported PyNPs. BbPyNP could catalyze the phosphorolysis of thymidine, 2'-deoxyuridine, uridine and 5-methyuridine. BbPyNP had good thermostability and retained 73% of its original activity after 2 h incubation at 50 A degrees C. BbPyNP had the highest activity at an optimum alkaline pH of 8.5. BbPyNP was stable from pH 7 to 9.8. Under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reached the yield of 61-84%.
An efficient approach was estimated in isolating thermostable PyNP from mesophilic bacteria.

• 出版日期2017-12
• 单位东南大学