科研之友
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摘要
In this study, a simple, rapid and sensitive high performance liquid chromatography (HPLC) method is developed for determination of tinidazole (TNZ) in human plasma samples using metronidazole as the internal standard (IS). Sample preparation was accomplished through protein precipitation with 70% perchloric acid, and chromatographic separation was carried out on a ZORBAX Eclipse XDB-C18 (4.6 x 250 mm, 5 mu m) at 40 degrees C. Mobile phase composed of a mixture of acetonitrile-2% glacial acetic acid-water (35: 40: 25) at a flow rate of 0.8 mL/min. Wavelength was set at 318 nm. The method was successfully applied to a bioequivalence study of oral TNZ drugs in Chinese healthy volunteers.
- 出版日期2014
- 单位温州医科大学
