Ascorbate-Glutathione Metabolism during PEG-Induced Water Deficit in Trifolium repens

作者：Wang, C. Q.^*; Liu, T.; Xu, H. J.

来源：Russian Journal of Plant Physiology, 2011, 58(4): 597-602.

DOI：10.1134/S1021443711040248

• Trifolium repens
• ascorbate peroxidase
• dehydroascorbate reductase
• glutathione reductase
• monodehydroascorbate reductase
• water deficit

In order to elucidate the response of the ascorbate-glutathione (ASC-GSH) cycle to drought stress, the activities of antioxidant enzymes and the levels of molecules involved in the ASC-GSH metabolism were studied in Trifolium repens L. seedlings subjected to PEG-induced water deficit. Compared to the control, the contents of H(2)O(2), thiobarbituric acid reactive substances (TBARS), ascorbate (ASC), dehydroascorbate (DHA), and glutathione disulfide (GSSG) increased in PEG-treated seedlings, whereas the glutathione (GSH) content kept constant during the drought period. Further more, the ASC/DHA and GSH/GSSG ratios decreased in the presence of PEG. Except for that of monodehydroascorbate reductase (MDHAR), the activities of ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), and glutathione reductase (GR) were up-regulated during water deficit, and the increases in APX and DHAR activities were much higher than those in GR activity. These data indicate that fluctuations in the ASC-GSH metabolism resulted from PEG treatment may have a positive effect on drought stress mitigation in T. repens.

• 出版日期2011-7
• 单位山东理工大学