Flavisolibacter ginsenosidimutans sp nov., with ginsenoside-converting activity isolated from soil used for cultivating ginseng

作者：Zhao, Yan; Liu, Qingmei; Kang, Myung-Suk; Jin, Fengxie; Yul, Hongshan^*; Im, Wan-Taek

来源：International Journal of Systematic and Evolutionary Microbiology, 2015, 65(12): 4868-4872.

DOI：10.1099/ijsem.0.000660

• GEN. NOV.

A Gram-reaction-negative, aerobic, non-motile and rod-shaped bacterial strain designated Gsoil 636(T) was isolated from soil of a ginseng cultivation field in Pocheon Province, South Korea and its taxonomic position was investigated using a polyphasic approach. Gsoil 636(T) grew at 18-30 degrees C and at pH 6.0-8.0 on R2A medium. Gsoil 636(T) possessed beta-glucosidase activity, which was responsible for its ability to transform ginsenoside Rb-1 (ones of the dominant active components of ginseng) to F-2. On the basis of 16S rRNA gene sequence similarity, Gsoil 636(T) was shown to belong to the family Chitinophagaceae and to be related to Flavisolibacter ginsengiterrae Gsoil 492(T) (96.7 % sequence similarity), Flavisolibacter ginsengisoli Gsoil 643(T) (96.6 %) and Flavisolibacter rigui 02SUJ3(T) (96.6 %). The G+C content of the genomic DNA was 48.9 % The predominant respiratory quinone was MK-7 and the major fatty acids were iso-C-15 (: 0), summed feature 3 (comprising C-16 (:) (1)omega 6c and/or C-16 (:) (1)omega 7c and iso-C-17 : (0) 3-OH. DNA and chemotaxonomic data supported the affiliation of Gsoil 636(T) to the genus Flavisolibacter. Gsoil 636(T) could be differentiated genotypically and phenotypically from the species of the genus Flavisolibacter with validly published names. The isolate therefore represents a novel species, for which the name Flavisolibacter ginsenosidimutans sp. nov. is proposed, with the type strain Gsoil 636(T) (KCTC 22818(T)=JCM 18197(T)=KACC 14277(T)).

• 出版日期2015-12
• 单位大连工业大学