Detection of Lumpy Skin Disease Virus Antigen and Genomic DNA in Formalin-Fixed Paraffin-Embedded Tissues from an Egyptian Outbreak in 2006

作者：Awadin W^*; Hussein H; Elseady Y; Babiuk S; Furuoka H

来源：Transboundary and Emerging Diseases, 2011, 58(5): 451-457.

DOI：10.1111/j.1865-1682.2011.01238.x

• lumpy skin disease virus
• formalin-fixed paraffin-embedded tissues
• Egypt

An outbreak of lumpy skin disease (LSD) was reported in 2006 in Egypt affecting 16 provinces. Biopsies and post-mortem tissue samples were collected from calves that showed typical clinical signs of LSD and fixed in formalin. These samples were collected from a private dairy farm in the Damietta province of Egypt. Formalin-fixed paraffin-embedded tissue samples were assessed using histology, and skin lesions were classified as either acute or subacute/chronic. Both lumpy skin disease virus (LSDV) DNA detected by polymerase chain reaction and LSDV antigen detected by immunohistochemistry using a capripoxvirus-specific monoclonal antibody were observed in the acute skin lesions and in some subacute/chronic skin lesions.

• 出版日期2011-10