Monitoring of HPLC profiles of selected polyphenolic compounds in sea buckthorn (HippophaA %26lt;%26lt; rhamnoides L.) plant parts during annual growth cycle and estimation of their antioxidant potential

作者：Bittova Miroslava^*; Krejzova Eliska; Roblova Vendula; Kuban Petr; Kuban Vlastimil

来源：CENTRAL EUROPEAN JOURNAL OF CHEMISTRY, 2014, 12(11): 1152-1161.

DOI：10.2478/s11532-014-0562-y

• Polyphenolic compounds
• Flavonoids
• Sea buckthorn
• Total antioxidant activity
• Total polyphenolic content

Presented work summarizes the data about polyphenolic profiles in various plant parts (leaves, shoots, berries) of sea buckthorn (HippophaA %26lt;%26lt; rhamnoides L.) during the annual growth cycle. A reversed-phase high performance liquid chromatography method (RP-HPLC) coupled with diode-array detection (DAD) was optimized for determination of catechin, epicatechin, gallic acid, p-coumaric acid, caffeic acid, ferulic acid, rutin (quercetin 3-rutinoside) and quercitrin (quercetin 3-rhamnoside). The content of these polyphenolic compounds was monitored in extracts of sea buckthorn plant samples from April to October. The total antioxidant activity was determined using scavenging of 2,2%26apos;-azino-bis(3-ethylbenzothiazoline-6-sulphonate) cation radical (ABTS(center dot+)) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH center dot). The total content of polyphenols was estimated by conventional spectrophotometric method using Folin-Ciocalteu reagent. The monitoring of temporal changes of selected polyphenolic compounds by RP-HPLC showed that catechin, epicatechin and gallic acid were the most abundant analytes in annual green shoots and leaves, and their content varied significantly during the studied period.

• 出版日期2014-11