Apolipoprotein D, apolipoprotein R and ST6GalNAc4 genes respond to clenbuterol administration in pig adipose tissue

作者：Zhang Jin^*; Lin Jiali; Shen Liangcai; Zhang Fan; Zhu Yingbo; Zheng Libin

来源：African Journal of Biotechnology, 2011, 10(39): 7577-7584.

• Pig
• clenbuterol
• adipose
• cDNA microarray
• real-time polymerase chain reaction (PCR)

Pig fat accumulation can be reduced by feeding pigs with high dosages of clenbuterol, but the molecular mechanism has not yet been well characterized. cDNA microarray and real-time PCR were used to identify the molecules potentially responding to clenbuterol in adipose cells. The mRNAs of 17 genes were found to be differentially expressed more than 2 fold (ratios of the expression levels in the test pigs to those in the control pigs of more than 2 or less than 0.5) by microarray analysis, including three lipid metabolism related genes (apolipoprotein D, apolipoprotein R and ST6GalNAc4). Eight genes were verified by real-time PCR, with six of them reproducing the result from microarray analyses. The results showed that apolipoprotein D, apolipoprotein R and ST6GalNAc4 genes respond to clenbuterol administration in pig adipose tissue and their functions may relate to fat accumulation reduction.

• 出版日期2011-7-27
• 单位河北科技师范学院