N-(p-coumaroyl) serotonin induces cell cycle arrest and apoptosis in breast cancer cells

作者：Mitsis Michail; Alexiou George A; Vartholomatos Evrysthenis; Markopoulos Georgios; Lazari Diamanto; Hodaj Entela; Nastos Dimitrios; Zagorianakou Panagiota; Galani Vasiliki; Kyritsis Athanasios P

来源：Journal of Buon, 2018, 23(1): 129-133.

• apoptosis
• breast cancer
• cell cycle
• N-(p-coumaroyl) serotonin

Purpose: Breast cancer is the most commonly diagnosed malignancy among women. Breast cancer cells may develop resistance to current chemotherapy, thus new chemotherapeutic agents are urgently needed.
Methods: A major number of drugs with anticancer activity have been isolated from plants. Herewith, we investigated for the first time the effect of N-(p-coumaroyl) serotonin (CS), isolated from Centaurea seed on a drug-resistant breast carcinoma (MCF-7) cells. Viability and proliferation of the cells were examined with trypan blue exclusion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Caspace-8, cell cycle, and CD24/CD44/CD56/CD58/CD71/CD15 expression were tested with flow cytometry.
Results: Treatment with CS significantly reduced cell viability. Induction of cell death and cell cycle arrest was confirmed with flow cytometry. After treatment with CS, there was a dose-dependent decrease in CD24/CD44/CD58/CD71 expression, whereas there was no change in CD56 and CD15 expression.
Conclusion: The treatment of breast cancer cells with CS may represent a novel therapeutic strategy and requires further investigation.

• 出版日期2018-2