Auxin and nutritional stress coupled somatic embryogenesis in Oldenlandia umbellata L.

作者：Krishnan S R Saranya; Siril E A^*

来源：Physiology and Molecular Biology of Plants, 2017, 23(2): 471-475.

DOI：10.1007/s12298-017-0425-z

• 2,4-D
• Nutritional stress
• Oldenlandia umbellata
• Plant regeneration
• Somatic embryos

Somatic embryos were induced from internodal segment derived callus of Oldenlandia umbellata L., in MS medium supplemented with different concentrations of 2,4-Dichlorophenoxy acetic acid (2,4-D). Initially calli were developed from internodes of microshoots inoculated in 2.5 mu M NAA supplemented medium. Then calli were transferred to 2,4-D added medium for somatic embryogenesis. Nutritional stress coupled with higher concentration of 2,4-D triggered somatic embryogenesis. Nutritional stress was induced by culturing callus in a fixed amount of medium for a period up to 20 weeks without any external supply of nutrients. Addition of 2.5 mu M 2,4-D gave 100% embryogenesis within 16 weeks of incubation. Callus mass bearing somatic embryos were transferred to germination medium facilitated production of in vitro plantlets. MS medium supplemented with 2.5 mu M benzyl adenine and 0.5 mu M alpha-naphthalene acetic acid produced 15.33 plants per culture within 4 weeks of culture. Somatic embryo germinated plants were then hardened and transferred to green house.

• 出版日期2017-4