A simple assay for 6-aminohexanoate-oligomer-hydrolase using N-(4-nitrophenyl)-6-aminohexanamide

作者：Taguchi Hisataka^*; Wakamatsu Makoto; Aso Kenji; Ono Shin; Shin Takashi; Akamatsu Takashi

来源：Journal of Bioscience and Bioengineering, 2012, 114(6): 586-588.

DOI：10.1016/j.jbiosc.2012.07.006

• 6-Aminohexanoate-linear-dimer hydrolase
• Chromogenic substrates
• Nylon-6
• K-m value
• Nylon oligomer

We synthesized N-(4-nitrophenyl)-6-aminohexanamide (AHpNA) and used it as a substrate in a kinetic study of 6-aminohexanoate-hydrolase (NylB), a nylon oligomer-hydrolyzing enzyme. NylBs derived from Arthrobacter sp. KI72 and Pseudomonas sp. NK87 hydrolyzed AHpNA as well as a 6-aminohexanoic acid dimer, a known substrate for NylB. The Kin values of the NylB from Arthrobacter sp. KI72 and Pseudomonas sp. NK87 for AHpNA were 0.5 mM and 2.0 mM, respectively.

• 出版日期2012-12