Molecular and functional interactions of cat APOBEC3 and feline foamy and immunodeficiency virus proteins: Different ways to counteract host-encoded restriction

作者：Chareza Sarah; Lukic Dragana Slavkovic; Liu Yang; Raethe Ann Mareen; Muenk Carsten; Zabogli Elisa; Pistello Mauro; Loechelt Martin

来源：Virology, 2012, 424(2): 138-146.

DOI：10.1016/j.virol.2011.12.017

• Foamy virus
• Feline immunodeficiency virus
• Anti-viral restriction
• APOBEC3 cytidine deaminase
• Bet protein
• Vif protein

Defined host-encoded feline APOBEC3 (feA3) cytidine deaminases efficiently restrict the replication and spread of exogenous retroviruses like Feline Immunodeficiency Virus (FIV) and Feline Foamy Virus (FFV) which developed different feA3 counter-acting strategies. Here we characterize the molecular interaction of FFV proteins with the diverse feA3 proteins. The FFV accessory protein Bet is the virus-encoded defense factor which is shown here to bind all feA3 proteins independent of whether they restrict FFV, a feature shared with FIV Vif that induces degradation of all feA3s including those that do not inactivate Fly. In contrast, only some feA3 proteins bind to FFV Gag, a pattern that in part reflects the restriction pattern detected. Additionally, one-domain feA3 proteins can homo- and hetero-dimerize in vitro, but a trans-dominant phenotype of any of the low-activity feA3 forms on FFV restriction by one of the highly-active feA3Z2 proteins was not detectable.

• 出版日期2012-3-15