Binding of human ribosomal protein p40 and its truncated mutants to the small ribosomal subunit

作者：Kossinova O A^*; Malygin A A; Babailova E S; Karpova G G

来源：Molecular Biology, 2008, 42(6): 911-916.

DOI：10.1134/S0026893308060125

• ribosomal protein SA (p40)
• laminin-binding domain
• 40S ribosomal subunit
• RNA-protein interaction

Ribosomal protein SA (rpSA), or p40, is a structural element of the small subunit of the eukaryotic ribosome. The N-terminal and central parts of rpSA are homologous to prokaryotic S2, whereas its C-terminal part is specific to eukaryotes. Preparations of 40S ribosomal subunits isolated from full-term human placenta proved to be deficient in SA to a varying extent. To study the rpSA binding to human 40S subunits, recombinant rpSA and its mutant forms with N-and C-terminal deletions were synthesized. The full-size and N-truncated rpSA variants bound to 40S subunits, while deletion of the C-terminal domain completely abolished the binding.

• 出版日期2008-12