An Electrochemical Method for High Sensitive Detection of Thiabendazole and Its Interaction with Human Serum Albumin

作者：Yang Jichun; Zhang Danfeng; Wang Lingling; Long Na; Zhang Minhui; Zhang Lei^*

来源：Food Analytical Methods, 2015, 8(2): 507-514.

DOI：10.1007/s12161-014-9919-2

• Thiabendazole
• Human serum albumin
• Carboxyl-functionalized multi-walled carbon nanotubes
• Interaction
• Differential pulse voltammetry

In this study, a simple and highly sensitive electroanalytical method for the determination of thiabendazole (TBZ) was developed using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) at carboxyl-functionalized multi-walled carbon nanotubes (MWCNT-COOH) modified glassy carbon electrode (MWCNT-COOH/GCE) in phosphate buffer solution (pH = 6.0). MWCNT-COOH/GCE exhibits an enhanced effectiveness for the redox of TBZ. This constructed biosensor exhibited two linear relationships with TBZ concentration range from 5.00 x 10(-7) to 4.95 x 10(-6) mol/L and 4.95 x 10(-6) to 9.80 x 10(-5) mol/L, respectively. The detection limit was 3.0 x 10(-7) mol/L (S/N = 3) and the relative standard deviation (R.S.D.) was lower than 4.2 % (n = 6). The proposed method was successfully applied to determine TBZ in real samples and the results were satisfactory. The MWCNT-COOH/GCE electrode showed good reproducibility, stability, and anti-interference. The electrochemistry method was also described for the evaluation of TBZ and human serum albumin (HSA) interaction. In the presence of HSA, the peak currents of TBZ decreased linearly due to the formation of a kind of electro-inactive complex. The binding constant between TBZ and HSA was obtained by DPV.

• 出版日期2015-2
• 单位辽宁大学