Alternative Fas-mediated cell death pathway is dependent on the different cleavage patterns of procaspase-8

作者：Li, Yufeng; Qi, Lei; Iwao, Atsushi; Kihira, Kentaro; Dida, Francis; Song, Zhenhu; Azuma, Eiichi; Komada, Yoshihiro^*

来源：Molecular and Cellular Biochemistry, 2009, 331(1-2): 231-238.

DOI：10.1007/s11010-009-0164-8

• Apoptosis
• Fas receptor
• Caspase-8
• Bid

It has been reported that mitochondria-independent or mitochondria-dependent (type I/II) Fas signaling pathways in leukemia cells depend on the amount of active caspase-8. However, Bid molecules, which could not be cleaved in type I cells, could be effectively cleaved by recombinant active caspase-8 in vitro. The cleavage of recombinant Bid by recombinant active caspase-8 could be blocked by anti-p10 and anti-p18 specific antibodies. Fas receptors could be similarly internalized into cytoplasm in type I and type II cells. Interestingly, p10 subunit of active caspase-8 could be detected in both type I and II cells, while p18 subunit of active caspase-8 could be detected only in type II cells but not in type I cells. These results demonstrated that p18 subunit was necessary for Bid cleavage and the mitochondria pathway might be dependent on the release of p18 subunit from active caspase-8.

• 出版日期2009-11
• 单位上海交通大学