A Single-Scan Inhomogeneity-Tolerant NMR Method for High-Resolution Two-Dimensional J-Resolved Spectroscopy

作者：Zhan, Haolin; Lin, Xiaoqing; Wei, Zhiliang; Ye, Qimiao; Cai, Shuhui; You, Xueqiu; Huang, Yuqing^*; Chen, Zhong^*

来源：IEEE Transactions on Biomedical Engineering, 2019, 66(6): 1559-1566.

DOI：10.1109/TBME.2018.2875797

• 2D homonuclear J-resolved spectra
• single scan
• NMR spectroscopy
• inhomogeneous magnetic fields
• biological detection

Objective: A robust and general single-scan NMR method, SGEN-J, is proposed for real-time recording high-resolution two-dimensional (2D) homonuclear J-resolved spectra under inhomogeneous magnetic fields. Methods: This proposed NMR method is designed based on the combination of a selective gradient encoding module to encode chemical shifts with spatial positions, and a J-modulation decoding module to reveal encoded structural information. Multi-band SGEN-J is further implemented to effectively enhance spectral sensitivity with a sustained tolerance of field inhomogeneity. Results: The SGEN-J provides an effective way to rapidly recover chemical shifts, J coupling constants, and multiplet patterns under inhomogeneous magnetic fields. Experiments on various chemical solutions were performed to demonstrate the feasibility and effectiveness of SGEN-J. Experiments on pig marrow tissues were performed to further investigate the applicability of SGEN-J to biological samples with intrinsic susceptibility variations. Conclusion: Based on intrinsic advantages, SGEN-J serves as a helpful complement to existing 2D Jresolved methodologies in molecular structure elucidation and biomedical study, and offer bright perspectives for real-time analyzing in vivo biological systems and monitoring in situ chemical reactions.

• 出版日期2019-6
• 单位厦门大学