A convenientmethod to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid

Talas Andras; Kulcsar Peter Istvan; Weinhardt Nora; Borsy Adrienn; Toth Eszter; Szebenyi Kornelia; Krausz Sarah Laura; Huszar Krisztina; Vida Istvan; Sturm Adam; Gordos Bianka; Hoffmann Orsolya Ivett; Bencsura Petra; Nyeste Antal; Ligeti Zoltan; Fodor Elfrieda; Welker Ervin

doi:10.1093/dnares/dsx029

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A convenientmethod to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid

作者：Talas Andras; Kulcsar Peter Istvan; Weinhardt Nora; Borsy Adrienn; Toth Eszter; Szebenyi Kornelia; Krausz Sarah Laura; Huszar Krisztina; Vida Istvan; Sturm Adam; Gordos Bianka; Hoffmann Orsolya Ivett; Bencsura Petra; Nyeste Antal; Ligeti Zoltan; Fodor Elfrieda; Welker Ervin

来源：DNA Research, 2017, 24(6): 609-621.

DOI：10.1093/dnares/dsx029