Antibiotic resistance and distribution of SodaCI, sopE, sefA genes among Salmonella enteric serotype Enteritidis isolates from poultry

作者：Ekateriniadou L V^*; Boukouvala E; Papi R M; Zdragas A; Giantzi V; Kyriakidis D A

来源：Journal of the Hellenic Veterinary Medical Society, 2015, 66(2): 70-79.

• S. enteritidis
• plasmid analysis
• sodCI
• sopE and sefA genes
• bla(TEM-1)
• bla(TEM-2) genes

Present work aimed to examine the antibiotic resistance of the Salmonella enterica serotype Enteritidis (SE) isolates from poultry, to study the plasmid-mediated ampicillin resistance and to detect and determine the distribution of sodCI, sopE and sefA genes. Thirty-five SE isolates from one-day chicks, layers and broilers were studied for susceptibility/resistance to sixteen antimicrobial agents; 23 (65.7%) of them showed resistance to ampicillin, 5 (14.3%) to ampicillin and tetracycline, 4 (11.45%) to tetracycline and 1 (2.9%) isolate showed multi-drug resistance. Ampicillin (Amp(R)) and ampicillin/tetracycline (Amp(R)Te(R)) resistance was easily transferred by conjugation, and all isolates except two possessed a common band. The molecular mass of the plasmid carrying ampicillin resistance was approximately determined at 41kb after DNA digestion with BamHI, HindIII EcoRI, EcoRV and Pstl restriction enzymes and ligation of EcoRI fragments to pET29c. For the detection of TEM-1 or/and TEM-2 beta-lactamases, two pairs of primers were used in a polymerase chain reaction (PCR). The PCR products showed the presence of bla(TEM-1) gene in all isolates. The presence of SodCI, sopE, sefA genes was also examined by PCR. Twenty-two (62,8%) isolates carried the sodCI gene, thirty-four (97,2%) isolates carried the sopE gene and all isolates carried the sefA fimbrial locus.

• 出版日期2015-6