Mutagenesis Studies of Human Cystathionine beta-Synthase: Residues Important for Heme Binding and Substrate Interactions

作者：Ozaki Shin ichi^*; Sakaguchi Chihori; Nakahara Akira; Yoshiya Masahiro

来源：Protein and Peptide Letters, 2010, 17(3): 351-355.

DOI：10.2174/092986610790780233

• Cystathionine
• hydrogen sulfide
• heme
• pyridoxal 5'-phosphate

Human cystathionine beta-synthase (CBS) is a pyridoxal 5'-phosphate (PLP) dependent hemoprotein, which catalyzes the condensation of serine and homocysteine. Our mutagenesis studies suggest that Arg-266 is important to sense structural changes in heme-binding site, and that Gln-222 as well as Tyr-223 are involved in interactions with substrates.

• 出版日期2010-3