Assessment of using Laponite (R) cross-linked poly(ethylene oxide) for controlled cell adhesion and mineralization

作者：Gaharwar Akhilesh K; Schexnailder Patrick J; Kline Benjamin P; Schmidt Gudrun^*

来源：Acta Biomaterialia, 2011, 7(2): 568-577.

DOI：10.1016/j.actbio.2010.09.015

• Poly(ethylene oxide)
• Layered silicate
• Laponite clay
• Nanocomposite
• Cell adhesion

The in vitro cytocompatibility of silicate (Laponite (R) clay) cross-linked poly(ethylene oxide) (PEO) nanocomposite films using MC3T3-E1 mouse preosteoblast cells was investigated while cell adhesion, spreading, proliferation and mineralization were assessed as a function of film composition. By combining the advantageous characteristics of PEO polymer (hydrophilic, prevents protein and cell adhesion) with those of a synthetic and layered silicate (charged, degradable and potentially bioactive) some of the physical and chemical properties of the resulting polymer nanocomposites could be controlled. Hydration, dissolution and mechanical properties were examined and related to cell adhesion. Overall, this feasibility study demonstrates the ability of using model Laponite cross-linked PEO nanocomposites to create bioactive scaffolds.

• 出版日期2011-2