Minimizing artifactual elevation of lipid peroxidation products (F-2-isoprostanes) in plasma during collection and storage

作者：Barden A E^*; Mas E; Croft K D; Phillips M; Mori T A

来源：Analytical Biochemistry, 2014, 449: 129-131.

DOI：10.1016/j.ab.2013.12.030

• Isoprostanes
• Lipid peroxidation
• Gas chromatography-mass spectrometry

F-2-isoprostanes (F-2-IsoP's) are reliable measures of in vivo lipid oxidation, but care is required to prevent artifactual elevation. We examined the effects of blood collection and storage on plasma F-2-IsoP's. Blood was collected into EDTA/butylated hydroxytoluene/reduced glutathione (EDTA/BHT/GSH) or EDTA, at 4 degrees C or room temperature. Plasma was stored at -20 or -80 degrees C for 1 or 6 months before F-2-IsoP's were assayed by GC-MS. The temperature of blood collection did not affect F-2-IsoP's. However, storage at -20 degrees C or collection into EDTA resulted in significant increases in F-2-IsoP's. Blood collection into EDTA/BHT/GSH and storage at -80 degrees C minimizes artifactual elevation of plasma F-2-IsoP's.

• 出版日期2014-3-15
• 单位河北医科大学