Amperometric glucose biosensors based on layer-by-layer assembly of chitosan and glucose oxidase on the Prussian blue-modified gold electrode

作者：Yin Bing; Yuan Ruo^*; Chai Yaqing; Chen Shihong; Cao Shurui; Xu Yang; Fu Ping

来源：Biotechnology Letters, 2008, 30(2): 317-322.

DOI：10.1007/s10529-007-9534-z

• chitosan
• glucose biosensor
• glucose oxidase
• layer-by-layer assembly
• Prussian blue

A glucose biosensor based on layer-by-layer (LBL) self-assembling of chitosan and glucose oxidase (GOD) on a Prussian blue film was developed. First, Prussian blue was deposited on a cleaned gold electrode then chitosan and GOD were assembled alternately to construct a multilayer film. The resulting amperometric glucose biosensor exhibited a fast response time (within 10 s) and a linear calibration range from 6 mu M to 1.6 mM with a detection limit of 3.1 mu M glucose (s/n = 3). With the low operating potential, the biosensor showed little interference to the possible interferents, including ascorbic acid, acetaminophen and uric acid, indicating an excellent selectivity.

• 出版日期2008-2
• 单位西南大学