A synthetic luciferin improves bioluminescence imaging in live mice

作者：Evans Melanie S; Chaurette Joanna P; Adams Spencer T Jr; Reddy Gadarla R; Paley Miranda A; Aronin Neil; Prescher Jennifer A; Miller Stephen C^*

来源：Nature Methods, 2014, 11(4): 393-+.

DOI：10.1038/NMETH.2839

Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI with existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin.

• 出版日期2014-4