Antioxidant polyphenols extracted from Avocado epicarp (Persea americana var. Hass) inhibit Helicobacter pylori urease

作者：Chavez Felipe^*; Aranda Mario; Garcia Apolinaria; Pastene Edgar

来源：Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas, 2011, 10(3): 265-280.

• Helicobacter pylori
• urease
• Agro-industrial waste
• functional foods
• polyphenols
• antioxidant capacity
• Persea sp.

Chronic infection by Helicobacter pylori produce chronic gastritis leading to other more severe pathologies as peptic ulcer and gastric adenocarcinoma. New anti-H. pylori agents has been found in natural products, particularly polyphenols. The inhibition of enzymes such us urease appears to be an interesting strategy by which polyphenols could limit the colonization by H. pylori. From the exocarp of Persea americana (avocado fruit), we obtain a procyanidin-rich extract with a 77% of gallic acid equivalents (GAE). Such procyanidins derived from epicatechin. with a mean degree of polymerization DPm = 6.10. The antioxidant capacity was assessed by different methods as TEAC-DPPH, TEAC-CUPRAC, TEAC-FRAP, TEAC-crocin. The extract shown inhibitory activity against H. pylori urease with an IC(50) = 1.02 mu g GAE/mL. In order to obtain clusters of procyanidins with different molecular weights, avocado peel extract was fractioned. A clear relation between the molecular size of procyanidins and their urease inhibitory activity was observed.

• 出版日期2011-5