Activity-Dependent A-to-I RNA Editing in Rat Cortical Neurons

作者：Sanjana Neville E^*; Levanon Erez Y; Hueske Emily A; Ambrose Jessica M; Li Jin Billy

来源：GENETICS, 2012, 192(1): 281-U569.

DOI：10.1534/genetics.112.141200

Changes in neural activity influence synaptic plasticity/scaling, gene expression, and epigenetic modifications. We present the first evidence that short-term and persistent changes in neural activity can alter adenosine-to-inosine (A-to-I) RNA editing, a post-transcriptional site-specific modification found in several neuron-specific transcripts. In rat cortical neuron cultures, activity-dependent changes in A-to-I RNA editing in coding exons are present after 6 hr of high potassium depolarization but not after 1 hr and require calcium entry into neurons. When treatments are extended from hours to days, we observe a negative feedback phenomenon: Chronic depolarization increases editing at many sites and chronic silencing decreases editing. We present several different modulations of neural activity that change the expression of different mRNA isoforms through editing.

• 出版日期2012-9