Dynamic kinetic capillary isoelectric focusing: A powerful tool for studying protein-DNA interactions

作者：Liu, Zhen^*; Drabovich, Andrei P.; Krylov, Sergey N.; Pawliszyn, Janusz

来源：Analytical Chemistry, 2007, 79(3): 1097-1100.

DOI：10.1021/ac061876c

• COLUMN IMAGING DETECTION
• SINGLE-STRANDED-DNA
• EQUILIBRIUM MIXTURES
• ELECTROPHORESIS
• BINDING

A new method called dynamic kinetic capillary isoelectric focusing (DK-CIEF) is presented for the study of protein-DNA interactions. The method is based on CIEF with laser-induced fluorescence-whole column imaging detection in which protein-DNA complexes are separated with spatial resolution while dissociations of the complexes are dynamically monitored using a CCD camera with temporal resolution. This method allows for the discrimination of different complexes and the measurement of the individual dissociation rate constants.

• 出版日期2007-2-1
• 单位南京大学