N-Ras Forms Dimers at POPC Membranes

作者：Gueldenhaupt Joern; Rudack Till; Bachler Peter; Mann Daniel; Triola Gemma; Waldmann Herbert; Koetting Carsten; Gerwert Klaus

来源：Biophysical Journal, 2012, 103(7): 1585-1593.

DOI：10.1016/j.bpj.2012.08.043

Ras is a central regulator of cellular signaling pathways. It is mutated in 20-30% of human tumors. To perform its function, Ras has to be bound to a membrane by a posttranslationally attached lipid anchor. Surprisingly, we identified here dimerization of membrane anchored Ras by combining attenuated total reflectance Fourier transform infrared spectroscopy, biomolecular simulations, and Forster resonance energy transfer experiments. By analyzing x-ray structural models and molecular-dynamics simulations, we propose a dimerization interface between alpha-helices 4 and 5 and the loop between beta 2 and beta 3. This seems to explain why the residues D47, E49, R135, R161, and R164 of this interface are influencing Ras signaling in cellular physiological experiments, although they are not positioned in the catalytic site. Dimerization could catalyze nanoclustering, which is well accepted for membrane-bound Ras. The interface could provide a new target for a seemingly novel type of small molecule interfering with signal transduction in oncogenic Ras mutants.

• 出版日期2012-10-3