A disposable evanescent wave fiber optic sensor coated with a molecularly imprinted polymer as a selective fluorescence probe

作者：Ton Xuan Anh; Acha Victor; Bonomi Paolo; Bui Bernadette Tse Sum^*; Haupt Karsten

来源：Biosensors and Bioelectronics, 2015, 64: 359-366.

DOI：10.1016/j.bios.2014.09.017

• Molecularly imprinted polymer
• Signaling monomer
• Evanescent wave fiber optic sensor
• Fluorescence
• Mycotoxin

We have developed a disposable evanescent wave fiber optic sensor by coating a molecularly imprinted polymer (MIP) containing a fluorescent signaling group on a 4-cm long polystyrene optical waveguide. The MIP is composed of a naphthalimide-based fluorescent monomer, which shows fluorescence enhancement upon binding with carboxyl-containing molecules. The herbicide 2,4-dichlorophenoxyacetic acid and the mycotoxin citrinin were used as model analytes. The coating of the MIP was either performed ex-situ, by dip-coating the fiber with MIP particles synthesized beforehand, or in-situ by evanescent-wave photopolymerization on the fiber. The sensing element was interrogated with a fiber-coupled spectrofluorimeter. The fiber optic sensor detects targets in the low nM range and exhibits specific and selective recognition over structural analogs and non-related carboxyl-containing molecules. This technology can be extended to other carboxyl-containing analytes, and to a broader spectrum of targets using different fluorescent monomers.

• 出版日期2015-2-15