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摘要
A strictly anaerobic bacterial strain, WK011(T), was isolated from a methanogenic reactor treating waste from cattle farms. The cells stained Gram-negative and were curved rods with a polar or subpolar flagellum. Spore formation was not observed. The optimum temperature for growth was 35 degrees C and the optimum pH was 6.7. Tests for oxidase, catalase and nitrate-reduction activities were negative. Hydrogen sulfide was produced. The strain fermented carbohydrates and produced acetate and propionate as major fermentation products. The genomic DNA G+C content was 41.7 mol%. The major cellular fatty acids were C-15:0, C-16:1 omega 9c and C-18:1 dimethylacetal. The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid. The most closely related species to strain WK011(T) on the basis of 16S rRNA gene sequences were Propionispira arboris and Zymophilus raffinosivorans (95.6% sequence similarity to the type strains of both species). It was shown by phylogenetic and phenotypic examination of the type strains of related species, including the second species of the genus Zymophilus, Zymophilus paucivorans, that the two genera should be combined and that the two species of the genus Zymophilus should be transferred to the genus Propionispira, as Propionispira raffinosivorans comb. nov. (type strain SH2(T)=ATCC 49691(T)=DSM 20765(T)) and Propionispira paucivorans comb. nov. (type strain AA1(T)=ATCC 49689(T)=DSM 20756(T)), with an emended description of the genus Propionispira. Based on differences in the phylogenetic and phenotypic characteristics of strain WK011(T) from those of closely related species, the novel species Propionispira arcuata sp. nov. is proposed to accommodate the strain. The type strain is WK011(T) (=JCM 16475(T)=DSM 22929(T)).
- 出版日期2014-10
