Amperometric immunosensor for the determination of alpha-1-fetoprotein based on multiwalled carbon nanotube-silver nanoparticle composite

作者：Che Xin; Yuan Ruo^*; Chai Yaqin; Li Jingjing; Song Zhongju; Wang Jinfen

来源：Journal of Colloid and Interface Science, 2010, 345(2): 174-180.

DOI：10.1016/j.jcis.2010.01.033

• Amperometric immunosensor
• Multiwalled carbon nanotube-silver
• composite
• MnO(2)
• alpha-1-Fetoprotein

A new amperometric immunosensor for the determination of alpha-1-fetoprotein (AFP) has been constructed. First, a multiwalled carbon nanotube-silver (MWNT-Ag) composite was modified on the surface of a glassy carbon electrode. Then, chitosan-MnO(2) (CS-MnO(2)) with excellent film forming ability was dipped onto the MWNT-Ag-modified electrode. Subsequently, gold nanoparticles were electrodeposited on the electrode to immobilize anti-AFP. The assembly processes were characterized with cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy. MWNT-Ag composite was characterized by transmission electron microscopy. X-ray photoelectron spectroscopy, and UV-vis spectroscopy. The system was optimized to realize a reliable determination of AFP in the range of 0.25-250 ng/ml with a detection limit of 0.08 ng/ml (S/N = 3). The proposed immunosensor showed a rapid and highly sensitive amperometric response to AFP with acceptable stability and reproducibility.

• 出版日期2010-5-15
• 单位西南大学